Thermosensitive hydrogels of poly(methyl vinyl ether-co-maleic anhydride) - Pluronic (R) F127 copolymers for controlled protein release

Esther Moreno, Juana Schwartz, Eneko Larrañeta, Paul A. Nguewa, Carmen Sanmartin, Maite Agueeros, Juan M. Irache, Socorro Espuelas*

*Corresponding author for this work

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Thermosensitive hydrogels are of a great interest due to their many biomedical and pharmaceutical applications. In this study, we synthesized a new series of random poly (methyl vinyl ether-co-maleic anhydride) (Gantrez (R) AN, GZ) and Pluronic (R) F127 (PF127) copolymers (GZ-PF127), that formed thermosensitive hydrogels whose gelation temperature and mechanical properties could be controlled by the molar ratio of GZ and PF127 polymers and the copolymer concentration in water. Gelation temperatures tended to decrease when the GZm/PF127 ratio increased. Thus, at a fixed GZm/PF127 value, sol-gel temperatures decreased at higher copolymer concentrations. Moreover, these hydrogels controlled the release of proteins such as bovine serum albumin (BSA) and recombinant recombinant kinetoplastid membrane protein of Leishmania (rKMP-11) more than the PF127 system. Toxicity studies carried out in J774.2 macrophages showed that cell viability was higher than 80%. Finally, histopathological analysis revealed that subcutaneous administration of low volumes of these hydrogels elicited a tolerable inflammatory response that could be useful to induce immune responses against the protein cargo in the development of vaccine adjuvants.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalInternational Journal of Pharmaceutics
Volume459
Issue number1-2
Early online date04 Dec 2013
DOIs
Publication statusPublished - 01 Jan 2014

Keywords

  • Thermosensitive hydrogels
  • Pluronics
  • Polyanhydride
  • Protein
  • Controlled release
  • DRUG-RELEASE
  • POLY(ANHYDRIDE) NANOPARTICLES
  • BIOADHESIVE PROPERTIES
  • TRIBLOCK COPOLYMERS
  • BLOCK-COPOLYMERS
  • IN-VITRO
  • DELIVERY
  • PACLITAXEL
  • DNA
  • DISSOLUTION

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