Localized surface plasmon resonance (LSPR) of metallic nanostructures is a unique phenomenon which controls light in sub-wavelength volumes and enhances the light–matter interactions. Traditionally, the excitation and measurement of LSPR requires bulky external light sources and efforts to scale down to nano-plasmonic devices predominantly rely on the miniaturization of system and associated accessories. Addressing this, the reported work discusses the generation and detection of LSPR in large-area nanostructured Au surface using frictional charges generated by tribo-electrification against a polydimethylsiloxane (PDMS) surface. We observe a complex interplay of the localized surface plasmons with frictional charges via concurrent spectroscopic and triboelectric measurements undertaken for the detection of bioconjugation in streptavidin-biotin complex. The obtained triboelectric and LSPR shift data, when subjected to multivariate principle component analysis highlights the strong correlation between the peak-to-peak voltage triboelectric response and the ωLSPR shift. We further undertook the fundamental studies on LSPR induced effects of plasmo-electric potential and hot-electron generation in nanostructured Au surfaces using photo-modulated Kelvin Probe Force Microscopy (KPFM). The demonstrated concept of electrification of plasmon resonance thus provides the underlying basis for the subsequent development of self-powered plasmonic-based sensors and opens new horizons for nanophotonic applications.