Toxicity assessment of xenobiotic contaminated groundwater using lux modified Pseudomonas fluorescens

Elaine M. Boyd; Kenneth Killham; Julian Wright; Stephen Rumford; Malcolm Hetheridge; Rob Cumming; Andrew A. Meharg

doi:10.1016/S0045-6535(97)00271-3

Toxicity assessment of xenobiotic contaminated groundwater using lux modified Pseudomonas fluorescens

Elaine M. Boyd, Kenneth Killham, Julian Wright, Stephen Rumford, Malcolm Hetheridge, Rob Cumming, Andrew A. Meharg

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

63 Citations (Scopus)

Abstract

A bacterial bioassay, suitable for rapid screening to assess the relative toxicity of xenobiotic contaminated groundwater has been developed. The quantitative bioassay utilizes a decline in luminescence of the lux marked soil bacterium Pseudomonas fluorescens on exposure to contaminated groundwaters from which effective concentration (EC) values can be assessed and compared. P. fluorescens was most sensitive to semi-volatile organics in groundwaters but there was no correlation between EC value and chemical content. The sensitivity and reproducibility of the P. fluorescens bioassay was compared with that of Microtox and results showed that mean EC₅₀ values for diluted ground water replicate samples were 20% and 18% respectively. This suggested that the P. fluorescens bioassay was as applicable to groundwater screening as the widely used Microtox bioassay.

Original language	English
Pages (from-to)	1967-1985
Number of pages	19
Journal	Chemosphere
Volume	35
Issue number	9
DOIs	https://doi.org/10.1016/S0045-6535(97)00271-3
Publication status	Published - Nov 1997

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title = "Toxicity assessment of xenobiotic contaminated groundwater using lux modified Pseudomonas fluorescens",

abstract = "A bacterial bioassay, suitable for rapid screening to assess the relative toxicity of xenobiotic contaminated groundwater has been developed. The quantitative bioassay utilizes a decline in luminescence of the lux marked soil bacterium Pseudomonas fluorescens on exposure to contaminated groundwaters from which effective concentration (EC) values can be assessed and compared. P. fluorescens was most sensitive to semi-volatile organics in groundwaters but there was no correlation between EC value and chemical content. The sensitivity and reproducibility of the P. fluorescens bioassay was compared with that of Microtox and results showed that mean EC50 values for diluted ground water replicate samples were 20% and 18% respectively. This suggested that the P. fluorescens bioassay was as applicable to groundwater screening as the widely used Microtox bioassay.",

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T1 - Toxicity assessment of xenobiotic contaminated groundwater using lux modified Pseudomonas fluorescens

AU - Boyd, Elaine M.

AU - Killham, Kenneth

AU - Wright, Julian

AU - Rumford, Stephen

AU - Hetheridge, Malcolm

AU - Cumming, Rob

AU - Meharg, Andrew A.

PY - 1997/11

Y1 - 1997/11

N2 - A bacterial bioassay, suitable for rapid screening to assess the relative toxicity of xenobiotic contaminated groundwater has been developed. The quantitative bioassay utilizes a decline in luminescence of the lux marked soil bacterium Pseudomonas fluorescens on exposure to contaminated groundwaters from which effective concentration (EC) values can be assessed and compared. P. fluorescens was most sensitive to semi-volatile organics in groundwaters but there was no correlation between EC value and chemical content. The sensitivity and reproducibility of the P. fluorescens bioassay was compared with that of Microtox and results showed that mean EC50 values for diluted ground water replicate samples were 20% and 18% respectively. This suggested that the P. fluorescens bioassay was as applicable to groundwater screening as the widely used Microtox bioassay.

AB - A bacterial bioassay, suitable for rapid screening to assess the relative toxicity of xenobiotic contaminated groundwater has been developed. The quantitative bioassay utilizes a decline in luminescence of the lux marked soil bacterium Pseudomonas fluorescens on exposure to contaminated groundwaters from which effective concentration (EC) values can be assessed and compared. P. fluorescens was most sensitive to semi-volatile organics in groundwaters but there was no correlation between EC value and chemical content. The sensitivity and reproducibility of the P. fluorescens bioassay was compared with that of Microtox and results showed that mean EC50 values for diluted ground water replicate samples were 20% and 18% respectively. This suggested that the P. fluorescens bioassay was as applicable to groundwater screening as the widely used Microtox bioassay.

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U2 - 10.1016/S0045-6535(97)00271-3

DO - 10.1016/S0045-6535(97)00271-3

M3 - Article

C2 - 9353912

SN - 0045-6535

VL - 35

SP - 1967

EP - 1985

JO - Chemosphere

JF - Chemosphere

IS - 9

ER -

Toxicity assessment of xenobiotic contaminated groundwater using lux modified Pseudomonas fluorescens

Abstract

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