Transcription-independent function of polycomb group protein PSC in cell cycle control

Adone Tielenius Kruythoff-Mohd Sarip; Anna Lagarou; Cecile M. Doyen; Jan A. Van Der Knaap; Ülkü Aslan; Karel Bezstarosti; Yasmin Yassin; Hugh W. Brock; Jeroen A. Demmers; C. Peter Verrijzer

doi:10.1126/science.1215927

Transcription-independent function of polycomb group protein PSC in cell cycle control

Adone Tielenius Kruythoff-Mohd Sarip, Anna Lagarou, Cecile M. Doyen, Jan A. Van Der Knaap, Ülkü Aslan, Karel Bezstarosti, Yasmin Yassin, Hugh W. Brock, Jeroen A. Demmers, C. Peter Verrijzer^*

^*Corresponding author for this work

School of Medicine, Dentistry and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Polycomb group (PcG) proteins control development and cell proliferation through chromatin-mediated transcriptional repression. We describe a transcription-independent function for PcG protein Posterior sex combs (PSC) in regulating the destruction of cyclin B (CYC-B). A substantial portion of PSC was found outside canonical PcG complexes, instead associated with CYC-B and the anaphase-promoting complex (APC). Cell-based experiments and reconstituted reactions established that PSC and Lemming (LMG, also called APC11) associate and ubiquitylate CYC-B cooperatively, marking it for proteosomal degradation. Thus, PSC appears to mediate both developmental gene silencing and posttranslational control of mitosis. Direct regulation of cell cycle progression might be a crucial part of the PcG system's function in development and cancer.

Original language	English
Pages (from-to)	744-747
Number of pages	4
Journal	Science
Volume	336
Issue number	6082
DOIs	https://doi.org/10.1126/science.1215927
Publication status	Published - 11 May 2012

ASJC Scopus subject areas

General Medicine
General

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{863216ddcfd346809e86e16624a44024,

title = "Transcription-independent function of polycomb group protein PSC in cell cycle control",

abstract = "Polycomb group (PcG) proteins control development and cell proliferation through chromatin-mediated transcriptional repression. We describe a transcription-independent function for PcG protein Posterior sex combs (PSC) in regulating the destruction of cyclin B (CYC-B). A substantial portion of PSC was found outside canonical PcG complexes, instead associated with CYC-B and the anaphase-promoting complex (APC). Cell-based experiments and reconstituted reactions established that PSC and Lemming (LMG, also called APC11) associate and ubiquitylate CYC-B cooperatively, marking it for proteosomal degradation. Thus, PSC appears to mediate both developmental gene silencing and posttranslational control of mitosis. Direct regulation of cell cycle progression might be a crucial part of the PcG system's function in development and cancer.",

author = "\{Tielenius Kruythoff-Mohd Sarip\}, Adone and Anna Lagarou and Doyen, \{Cecile M.\} and \{Van Der Knaap\}, \{Jan A.\} and {\"U}lk{\"u} Aslan and Karel Bezstarosti and Yasmin Yassin and Brock, \{Hugh W.\} and Demmers, \{Jeroen A.\} and Verrijzer, \{C. Peter\}",

year = "2012",

month = may,

day = "11",

doi = "10.1126/science.1215927",

language = "English",

volume = "336",

pages = "744--747",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6082",

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TY - JOUR

T1 - Transcription-independent function of polycomb group protein PSC in cell cycle control

AU - Tielenius Kruythoff-Mohd Sarip, Adone

AU - Lagarou, Anna

AU - Doyen, Cecile M.

AU - Van Der Knaap, Jan A.

AU - Aslan, Ülkü

AU - Bezstarosti, Karel

AU - Yassin, Yasmin

AU - Brock, Hugh W.

AU - Demmers, Jeroen A.

AU - Verrijzer, C. Peter

PY - 2012/5/11

Y1 - 2012/5/11

N2 - Polycomb group (PcG) proteins control development and cell proliferation through chromatin-mediated transcriptional repression. We describe a transcription-independent function for PcG protein Posterior sex combs (PSC) in regulating the destruction of cyclin B (CYC-B). A substantial portion of PSC was found outside canonical PcG complexes, instead associated with CYC-B and the anaphase-promoting complex (APC). Cell-based experiments and reconstituted reactions established that PSC and Lemming (LMG, also called APC11) associate and ubiquitylate CYC-B cooperatively, marking it for proteosomal degradation. Thus, PSC appears to mediate both developmental gene silencing and posttranslational control of mitosis. Direct regulation of cell cycle progression might be a crucial part of the PcG system's function in development and cancer.

AB - Polycomb group (PcG) proteins control development and cell proliferation through chromatin-mediated transcriptional repression. We describe a transcription-independent function for PcG protein Posterior sex combs (PSC) in regulating the destruction of cyclin B (CYC-B). A substantial portion of PSC was found outside canonical PcG complexes, instead associated with CYC-B and the anaphase-promoting complex (APC). Cell-based experiments and reconstituted reactions established that PSC and Lemming (LMG, also called APC11) associate and ubiquitylate CYC-B cooperatively, marking it for proteosomal degradation. Thus, PSC appears to mediate both developmental gene silencing and posttranslational control of mitosis. Direct regulation of cell cycle progression might be a crucial part of the PcG system's function in development and cancer.

UR - https://www.scopus.com/pages/publications/84860834822

U2 - 10.1126/science.1215927

DO - 10.1126/science.1215927

M3 - Article

C2 - 22491092

AN - SCOPUS:84860834822

SN - 0036-8075

VL - 336

SP - 744

EP - 747

JO - Science

JF - Science

IS - 6082

ER -

Transcription-independent function of polycomb group protein PSC in cell cycle control

Abstract

ASJC Scopus subject areas

UN SDGs

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