TY - JOUR
T1 - Transcriptional regulation of a brown adipocyte-specific gene, UCP1, by KLF11 and KLF15
AU - Yamamoto, Ken-ichi
AU - Sakaguchi, Masakiyo
AU - Medina, Reinhold J
AU - Niida, Aya
AU - Sakaguchi, Yoshihiko
AU - Miyazaki, Masahiro
AU - Kataoka, Ken
AU - Huh, Nam-ho
N1 - Copyright © 2010 Elsevier Inc. All rights reserved.
PY - 2010/9
Y1 - 2010/9
N2 - Several growth factors and transcription factors have been reported to play important roles in brown adipocyte differentiation and modulation of thermogenic gene expression, especially the expression of UCP1. In this study, we focused on KLF11 and KLF15, which were expressed highly in brown adipose tissue. Our data demonstrated that KLF11 and KLF15 interacted directly with the UCP1 promoter using GC-box and GT-boxes, respectively. Co-transfection of KLF11 and KLF15 in the mesenchymal stem cell line muBM3.1 during brown adipocyte differentiation enhanced the expression level of UCP1. KLF11, but not KLF15, was essential for UCP1 expression during brown adipocyte differentiation of muBM3.1.
AB - Several growth factors and transcription factors have been reported to play important roles in brown adipocyte differentiation and modulation of thermogenic gene expression, especially the expression of UCP1. In this study, we focused on KLF11 and KLF15, which were expressed highly in brown adipose tissue. Our data demonstrated that KLF11 and KLF15 interacted directly with the UCP1 promoter using GC-box and GT-boxes, respectively. Co-transfection of KLF11 and KLF15 in the mesenchymal stem cell line muBM3.1 during brown adipocyte differentiation enhanced the expression level of UCP1. KLF11, but not KLF15, was essential for UCP1 expression during brown adipocyte differentiation of muBM3.1.
U2 - 10.1016/j.bbrc.2010.08.039
DO - 10.1016/j.bbrc.2010.08.039
M3 - Article
C2 - 20709022
SN - 0006-291X
VL - 400
SP - 175
EP - 180
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -