Tylosin Detection in Animal Feed by Liquid Chromatography-Tandem Mass Spectrometry with Enzymatic Hydrolysis of the Tylosin Urea Adduct

Christof Van Poucke*, Fréderic Dumoulin, Kirsten De Keyser, Chris Elliott, Carlos Van Peteghem

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

When the use of tylosin as a feed additive was forbidden by Council Regulation 2821/98, the necessity of a chemical confirmation method for the monitoring of the ban was created. Recently a method was developed for the detection of tylosin in animal feed by means of LC-MS/MS. During the validation high deviating values for the decision limit, detection capability, and repeatability for tylosin in cattle feed were observed, and the presence of urea and the formation of a tylosin urea adduct (TUA) were suggested as possible explanations. In this study two hydrolysis approaches for the TUA adduct were compared, namely, a chemical hydrolysis and an enzymatic hydrolysis with urease. The latter yielded a more complete hydrolysis of urea and was used for further validation. The recovery increased by ∼15-25% depending on the amount of urea present in the feed (0.5-2%). The decision limit and detection capability were hardly influenced by the enzymatic hydrolysis.

Original languageEnglish
Pages (from-to)2803-2806
Number of pages4
JournalJournal of Agricultural and Food Chemistry
Volume52
Issue number10
DOIs
Publication statusPublished - 19 May 2004

Keywords

  • Animal feed
  • LC-MS/MS
  • Tylosin
  • Urea adduct

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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