Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to α-glucosidase activity detection

Di Wu; Na Hu; Jichao Liu; Guangsen Fan; Xiuting Li; Jing Sun; Chunji Dai; Yourui Suo; Guoliang Li; Yongning Wu

doi:10.1016/j.talanta.2018.07.073

Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to α-glucosidase activity detection

Di Wu, Na Hu, Jichao Liu, Guangsen Fan, Xiuting Li, Jing Sun, Chunji Dai, Yourui Suo, Guoliang Li^*, Yongning Wu

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Recently, exploitation of nanozymes for signal amplification has aroused extensive research interesting in the fields of analytical chemistry and nanoscience. Herein, we introduced a new sensing strategy based on the ascorbic acid (AA) precisely regulated Ag₃PO₄ nanozyme for signal amplification. AA can reduce the partial Ag⁺ on the surface of Ag₃PO₄ nano-particles to form Ag° particles and result in the formation of Ag°/Ag₃PO₄ heterostructure. Due to surface plasmon resonance (SPR), the Ag° particles in heterostructure can act as the co-catalysts to enhance the electron-hole separation and the interfacial charge transfer, leading to a highly efficient oxidase mimicking activity for catalyzing the oxidation of the substrate of 3,3,5,5-tetramethylbenzidine (TMB) under visible light. Impressively, the activity enhancement of Ag₃PO₄ nano-peroxidase was linearly depended on the AA concentrations. Based on this feature, we employed it for multiple biological detections. For example, L-ascorbic acid-2-O-α-D-glucopyranosyl (AAG) was designed for α-glucosidase substrate, which can be hydrolyzed by α-glucosidase and lead to AA release. Further, the signal of α-glucosidase activity can be efficiently enlarged by Ag₃PO₄ nanozyme. Taking advantages of the powerful signal amplification by Ag₃PO₄ nanozymes, we developed the multiple sensing assays for monitoring AA in rat brain microdialysates, detection of α-glucosidase activity and its inhibitors (anti-diabetic drugs). The assay allowed the ultrasensitive colorimetric detection of α-glucosidase inhibitor with an ultra-low detection limit of 10 nM, and a naked-eye detection of the inhibitor concentration as low as 1 μM. The sensing strategy based on AA precisely regulated Ag₃PO₄ nano-peroxidase provides a promising candidate platform for highly stable and efficient cascaded signal amplification in biosensing field.

Original language	English
Pages (from-to)	103-109
Number of pages	7
Journal	Talanta
Volume	190
Early online date	29 Jul 2018
DOIs	https://doi.org/10.1016/j.talanta.2018.07.073
Publication status	Published - 01 Dec 2018
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by The National Natural Science Foundation of China ( 21677085 , 21537001 , and 81472986 ), the Natural Science Foundation of Shandong Province ( ZR2017JL012 ), the Open Project Fund of Beijing Advanced Innovation Center for Food Nutrition and Human Health ( 20181030 ), the Project funded by China Postdoctoral Science Foundation ( 2016M590071 ), and the Development Project of Qinghai Key Laboratory ( 2017-ZJ-Y10 ).

Publisher Copyright:
© 2018

Keywords

Inhibitor screening
Nanozymes
Photo-nanoperoxidase
α-glucosidase

ASJC Scopus subject areas

Analytical Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to α-glucosidase activity detection",

abstract = "Recently, exploitation of nanozymes for signal amplification has aroused extensive research interesting in the fields of analytical chemistry and nanoscience. Herein, we introduced a new sensing strategy based on the ascorbic acid (AA) precisely regulated Ag3PO4 nanozyme for signal amplification. AA can reduce the partial Ag+ on the surface of Ag3PO4 nano-particles to form Ag° particles and result in the formation of Ag°/Ag3PO4 heterostructure. Due to surface plasmon resonance (SPR), the Ag° particles in heterostructure can act as the co-catalysts to enhance the electron-hole separation and the interfacial charge transfer, leading to a highly efficient oxidase mimicking activity for catalyzing the oxidation of the substrate of 3,3,5,5-tetramethylbenzidine (TMB) under visible light. Impressively, the activity enhancement of Ag3PO4 nano-peroxidase was linearly depended on the AA concentrations. Based on this feature, we employed it for multiple biological detections. For example, L-ascorbic acid-2-O-α-D-glucopyranosyl (AAG) was designed for α-glucosidase substrate, which can be hydrolyzed by α-glucosidase and lead to AA release. Further, the signal of α-glucosidase activity can be efficiently enlarged by Ag3PO4 nanozyme. Taking advantages of the powerful signal amplification by Ag3PO4 nanozymes, we developed the multiple sensing assays for monitoring AA in rat brain microdialysates, detection of α-glucosidase activity and its inhibitors (anti-diabetic drugs). The assay allowed the ultrasensitive colorimetric detection of α-glucosidase inhibitor with an ultra-low detection limit of 10 nM, and a naked-eye detection of the inhibitor concentration as low as 1 μM. The sensing strategy based on AA precisely regulated Ag3PO4 nano-peroxidase provides a promising candidate platform for highly stable and efficient cascaded signal amplification in biosensing field.",

keywords = "Inhibitor screening, Nanozymes, Photo-nanoperoxidase, α-glucosidase",

author = "Di Wu and Na Hu and Jichao Liu and Guangsen Fan and Xiuting Li and Jing Sun and Chunji Dai and Yourui Suo and Guoliang Li and Yongning Wu",

note = "Funding Information: This work was supported by The National Natural Science Foundation of China ( 21677085 , 21537001 , and 81472986 ), the Natural Science Foundation of Shandong Province ( ZR2017JL012 ), the Open Project Fund of Beijing Advanced Innovation Center for Food Nutrition and Human Health ( 20181030 ), the Project funded by China Postdoctoral Science Foundation ( 2016M590071 ), and the Development Project of Qinghai Key Laboratory ( 2017-ZJ-Y10 ). Publisher Copyright: {\textcopyright} 2018",

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month = dec,

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doi = "10.1016/j.talanta.2018.07.073",

language = "English",

volume = "190",

pages = "103--109",

journal = "Talanta",

issn = "0039-9140",

publisher = "Elsevier B.V.",

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T1 - Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to α-glucosidase activity detection

AU - Wu, Di

AU - Hu, Na

AU - Liu, Jichao

AU - Fan, Guangsen

AU - Li, Xiuting

AU - Sun, Jing

AU - Dai, Chunji

AU - Suo, Yourui

AU - Li, Guoliang

AU - Wu, Yongning

N1 - Funding Information: This work was supported by The National Natural Science Foundation of China ( 21677085 , 21537001 , and 81472986 ), the Natural Science Foundation of Shandong Province ( ZR2017JL012 ), the Open Project Fund of Beijing Advanced Innovation Center for Food Nutrition and Human Health ( 20181030 ), the Project funded by China Postdoctoral Science Foundation ( 2016M590071 ), and the Development Project of Qinghai Key Laboratory ( 2017-ZJ-Y10 ). Publisher Copyright: © 2018

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Recently, exploitation of nanozymes for signal amplification has aroused extensive research interesting in the fields of analytical chemistry and nanoscience. Herein, we introduced a new sensing strategy based on the ascorbic acid (AA) precisely regulated Ag3PO4 nanozyme for signal amplification. AA can reduce the partial Ag+ on the surface of Ag3PO4 nano-particles to form Ag° particles and result in the formation of Ag°/Ag3PO4 heterostructure. Due to surface plasmon resonance (SPR), the Ag° particles in heterostructure can act as the co-catalysts to enhance the electron-hole separation and the interfacial charge transfer, leading to a highly efficient oxidase mimicking activity for catalyzing the oxidation of the substrate of 3,3,5,5-tetramethylbenzidine (TMB) under visible light. Impressively, the activity enhancement of Ag3PO4 nano-peroxidase was linearly depended on the AA concentrations. Based on this feature, we employed it for multiple biological detections. For example, L-ascorbic acid-2-O-α-D-glucopyranosyl (AAG) was designed for α-glucosidase substrate, which can be hydrolyzed by α-glucosidase and lead to AA release. Further, the signal of α-glucosidase activity can be efficiently enlarged by Ag3PO4 nanozyme. Taking advantages of the powerful signal amplification by Ag3PO4 nanozymes, we developed the multiple sensing assays for monitoring AA in rat brain microdialysates, detection of α-glucosidase activity and its inhibitors (anti-diabetic drugs). The assay allowed the ultrasensitive colorimetric detection of α-glucosidase inhibitor with an ultra-low detection limit of 10 nM, and a naked-eye detection of the inhibitor concentration as low as 1 μM. The sensing strategy based on AA precisely regulated Ag3PO4 nano-peroxidase provides a promising candidate platform for highly stable and efficient cascaded signal amplification in biosensing field.

AB - Recently, exploitation of nanozymes for signal amplification has aroused extensive research interesting in the fields of analytical chemistry and nanoscience. Herein, we introduced a new sensing strategy based on the ascorbic acid (AA) precisely regulated Ag3PO4 nanozyme for signal amplification. AA can reduce the partial Ag+ on the surface of Ag3PO4 nano-particles to form Ag° particles and result in the formation of Ag°/Ag3PO4 heterostructure. Due to surface plasmon resonance (SPR), the Ag° particles in heterostructure can act as the co-catalysts to enhance the electron-hole separation and the interfacial charge transfer, leading to a highly efficient oxidase mimicking activity for catalyzing the oxidation of the substrate of 3,3,5,5-tetramethylbenzidine (TMB) under visible light. Impressively, the activity enhancement of Ag3PO4 nano-peroxidase was linearly depended on the AA concentrations. Based on this feature, we employed it for multiple biological detections. For example, L-ascorbic acid-2-O-α-D-glucopyranosyl (AAG) was designed for α-glucosidase substrate, which can be hydrolyzed by α-glucosidase and lead to AA release. Further, the signal of α-glucosidase activity can be efficiently enlarged by Ag3PO4 nanozyme. Taking advantages of the powerful signal amplification by Ag3PO4 nanozymes, we developed the multiple sensing assays for monitoring AA in rat brain microdialysates, detection of α-glucosidase activity and its inhibitors (anti-diabetic drugs). The assay allowed the ultrasensitive colorimetric detection of α-glucosidase inhibitor with an ultra-low detection limit of 10 nM, and a naked-eye detection of the inhibitor concentration as low as 1 μM. The sensing strategy based on AA precisely regulated Ag3PO4 nano-peroxidase provides a promising candidate platform for highly stable and efficient cascaded signal amplification in biosensing field.

KW - Inhibitor screening

KW - Nanozymes

KW - Photo-nanoperoxidase

KW - α-glucosidase

U2 - 10.1016/j.talanta.2018.07.073

DO - 10.1016/j.talanta.2018.07.073

M3 - Article

C2 - 30172485

AN - SCOPUS:85050563907

VL - 190

SP - 103

EP - 109

JO - Talanta

JF - Talanta

SN - 0039-9140

ER -

Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to α-glucosidase activity detection

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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