Understanding the evolution and metabolic capabilities of the Butyrivibrio group

Sara Pidcock, Timofey Skvortsov, Fernanda Godoy Santos, Karen Siu-Ting, Christopher Creevey, Sharon Huws

Research output: Contribution to conferencePoster


Exploring and understanding the phylogeny of the Butyrivibrio group is imperative if we are ever to fully understand the consortium of ruminal microbial enzymes that are responsible for the catalysis of multifaceted reactions, such as biohydrogenation.
At present, taxonomic classification of the Butyrivibrio group is based primarily on butyrate production. This approach has become antiquated with the development of sequencing technologies and downstream bioinformatics analysis. This study investigated the taxonomic relatedness and functional capacity of the ruminal Butyrivibrio group using 72 genomes. Seventy-one Butyrivibrio group genomes were obtained via JGI (the Hungate 1000 project), and one additional bacterial strain was sequenced by ourselves. A 40 marker phylogenetic tree was constructed and visualised with the interactive Tree Of Life (iTOL), and pangenome analysis conducted using Spine/ClustAGE. Orthologous gene affiliations were identified using OrthAgogue, and glycosyl hydrolase families were identified using dbCAN then aligned with Clustal Omega. Data obtained showed that three primary clades were observed, namely the genus Pseudobutyrivibrio, B. fibrisolvens, and the remaining Butyrivibrio species. Pangenome analysis and orthologous gene affiliations revealed greater diversity within Butyrivibrio than Pseudobutyrivibrio. Butyrivibrio clades consistently showed smaller core genome sizes in comparison to Pseudobutyrivibrio, with core genome percentages as low as 4%, indicating high levels of variance. Glycosyl hydrolase alignment shows extensive sequence dissimilarity between genes on a nucleotide and amino acid level These findings suggest that the Butyrivibrio group are highly evolved to maintain competitiveness in the rumen and emphasises the need for further research into the biochemical capacity of the Butyrivibrio group.
Original languageEnglish
Number of pages1
Publication statusPublished - 2019
EventMicrobiology Society Annual Conference 2019 - Belfast, Belfast, United Kingdom
Duration: 08 Apr 201911 Apr 2019


ConferenceMicrobiology Society Annual Conference 2019
Country/TerritoryUnited Kingdom


Dive into the research topics of 'Understanding the evolution and metabolic capabilities of the Butyrivibrio group'. Together they form a unique fingerprint.

Cite this