Universal plastid primers for chlorophyta and rhodophyta

Jim Provan, S. Murphy, Christine Maggs

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

To date, the majority of molecular genetic studies in algae have utilized a fairly limited range of markers such as the plastid rbcL gene and spacer, the mitochondrial cox2-3 spacer or the nuclear ribosomal DNA and spacers. The lack of available markers has been particularly problematic in studies of within-species variation. Whilst microsatellites are now being developed in many algal species, there remains a need for universal markers that can be applied to a wide range of species. The increasing availability of complete plastid genome sequences for several algae has allowed us to develop two sets of universal primers, similar to those available in higher plants, for the amplification of coding and non-coding regions of the plastid genome in red and green algae. These markers are expected to be useful in a broad range of algal population genetic and phylogenetic studies.
Original languageEnglish
Pages (from-to)43-50
Number of pages8
JournalEuropean Journal of Phycology
Volume39(1)
Issue number1
DOIs
Publication statusPublished - Feb 2004

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

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