Universal primers for the amplification of chloroplast microsatellites in grasses (Poaceae)

Jim Provan, P.M. Biss, D. McMeel, S. Mathews

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Attempts to design truly universal primers to amplify chloroplast microsatellites have met with limited success due to nonconservation of repeat loci across widely divergent taxa. We have used the complete chloroplast genome sequences of rice, maize and wheat to design five pairs of primers that amplify homologous mononucleotide repeats across the Poaceae (grasses). Sequencing confirmed conservation of repeat motifs across subfamilies and a preliminary study in Anthoxanthum odoratum revealed polymorphism at two loci with a haplotype diversity value of 0.495. These primers provide a valuable tool to study cytoplasmic diversity in this extensively studied and economically important range of taxa.
Original languageEnglish
Pages (from-to)262-264
Number of pages3
JournalMOLECULAR ECOLOGY NOTES
Volume4
Issue number2
Publication statusPublished - Jun 2004

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Ecology

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    Provan, J., Biss, P. M., McMeel, D., & Mathews, S. (2004). Universal primers for the amplification of chloroplast microsatellites in grasses (Poaceae). MOLECULAR ECOLOGY NOTES, 4(2), 262-264.