Unusual, stable replicating viruses generated from mumps virus cDNA clones

Connor Bamford, Elizabeth Wignall-Fleming, Vattipally B Sreenu, Richard Randall, Paul Duprex, Bertus Rima

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Abstract

In reverse genetic experiments we have isolated recombinant mumps viruses (rMuV) that carry large numbers of mutations clustered in small parts of their genome, which are not caused by biased hyper-mutation. In two separate experiments we obtained such recombinant viruses: one virus had 11 mutations in the V/P region of the genome; the other, which also contained an extra transcription unit encoding green fluorescent protein (EGFP), had 32 mutations in the N gene. These specific sets of mutations have not been observed in naturally occurring MuV isolates. Unusually, the vast majority of the mutations (48/51) were synonymous. On passage in Vero cells and human B-LCL cells, a B lymphocyte-like cell line, these mutations appear stable as no reversion occurred to the original consensus sequence, although mutations in other parts of the genome occurred and changed in frequency during passage. Defective interfering RNAs accumulate in passage in Vero cells but not in B-LCL cells. Interestingly, in all passaged samples the level of variation in the EGFP gene is the same as in the viral genes, though it is unlikely that this gene is under any functionality constraint. What mechanism gave rise to these viruses with clustered mutations and their stability remains an open question, which is likely of interest to a wider field than mumps reverse genetics.

Original languageEnglish
Pages (from-to)e0219168
JournalPLoS ONE
Volume14
Issue number7
DOIs
Publication statusPublished - 05 Jul 2019

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Mumps virus
Viruses
Complementary DNA
Clone Cells
Genes
clones
mutation
viruses
Mutation
B-lymphocytes
Green Fluorescent Proteins
Reverse Genetics
Vero Cells
Genome
green fluorescent protein
genome
genes
Lymphocytes
Transcription
Mumps

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Bamford, C., Wignall-Fleming, E., Sreenu, V. B., Randall, R., Duprex, P., & Rima, B. (2019). Unusual, stable replicating viruses generated from mumps virus cDNA clones. PLoS ONE, 14(7), e0219168. https://doi.org/10.1371/journal.pone.0219168
Bamford, Connor ; Wignall-Fleming, Elizabeth ; Sreenu, Vattipally B ; Randall, Richard ; Duprex, Paul ; Rima, Bertus. / Unusual, stable replicating viruses generated from mumps virus cDNA clones. In: PLoS ONE. 2019 ; Vol. 14, No. 7. pp. e0219168.
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Bamford, C, Wignall-Fleming, E, Sreenu, VB, Randall, R, Duprex, P & Rima, B 2019, 'Unusual, stable replicating viruses generated from mumps virus cDNA clones', PLoS ONE, vol. 14, no. 7, pp. e0219168. https://doi.org/10.1371/journal.pone.0219168

Unusual, stable replicating viruses generated from mumps virus cDNA clones. / Bamford, Connor; Wignall-Fleming, Elizabeth; Sreenu, Vattipally B; Randall, Richard; Duprex, Paul; Rima, Bertus.

In: PLoS ONE, Vol. 14, No. 7, 05.07.2019, p. e0219168.

Research output: Contribution to journalArticle

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Bamford C, Wignall-Fleming E, Sreenu VB, Randall R, Duprex P, Rima B. Unusual, stable replicating viruses generated from mumps virus cDNA clones. PLoS ONE. 2019 Jul 5;14(7):e0219168. https://doi.org/10.1371/journal.pone.0219168