Uptake and accumulation of Microcystin-LR based on exposure through drinking water: An animal model assessing the human health risk

Brett Greer*, Julie P. Meneely, Christopher T. Elliott

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Citations (Scopus)
105 Downloads (Pure)

Abstract

Harmful Algal Blooms (HABs) in freshwater systems and intensified aquaculture have increased the risk to human health through exposure to cyanotoxins such as microcystin-LR (MC-LR). To understand the uptake and processing of MC-LR in humans, the pig was chosen as an animal model. This was assessed by repeated exposure for 13 weeks of eight animals dosed daily with MC-LR at 0.04 μg/kg bw, repeated with six animals over five weeks at a dose 50 times higher at 2 μg/kg bw. An analytical method was developed for MC-LR in porcine serum and also to analyse levels of free MC-LR in harvested porcine tissues, with Lemieux Oxidation employed to determine bound MC-LR in these tissues. MC-LR was not detected in the serum of treated animals from either experiment but free MC-LR was observed in the large intestine and kidney from two animals from the higher dosed group at levels of 1.4 and 1.9 μg/kg dry weight (dw) respectively. The results indicated 50% of higher dosed animals accumulated bound MC-LR in liver tissue, averaging 26.4 μg, approximately 1.1% of the dose administered. These results point to the potential uptake and accumulation of MC-LR in human liver tissue exposed chronically to sub-acute doses.

Original languageEnglish
Article number4913
Number of pages10
JournalScientific Reports
Volume8
Issue number1
Early online date20 Mar 2018
DOIs
Publication statusEarly online date - 20 Mar 2018

Fingerprint

microcystin-LR
drinking water
human health
animal models
uptake mechanisms
animals
swine
cyanobacterial toxins
liver
administered dose
large intestine
dosage
algal blooms
analytical methods
aquaculture
kidneys
oxidation

Cite this

@article{40ab13fb035b4ece9ce91e6c8e172afb,
title = "Uptake and accumulation of Microcystin-LR based on exposure through drinking water: An animal model assessing the human health risk",
abstract = "Harmful Algal Blooms (HABs) in freshwater systems and intensified aquaculture have increased the risk to human health through exposure to cyanotoxins such as microcystin-LR (MC-LR). To understand the uptake and processing of MC-LR in humans, the pig was chosen as an animal model. This was assessed by repeated exposure for 13 weeks of eight animals dosed daily with MC-LR at 0.04 μg/kg bw, repeated with six animals over five weeks at a dose 50 times higher at 2 μg/kg bw. An analytical method was developed for MC-LR in porcine serum and also to analyse levels of free MC-LR in harvested porcine tissues, with Lemieux Oxidation employed to determine bound MC-LR in these tissues. MC-LR was not detected in the serum of treated animals from either experiment but free MC-LR was observed in the large intestine and kidney from two animals from the higher dosed group at levels of 1.4 and 1.9 μg/kg dry weight (dw) respectively. The results indicated 50{\%} of higher dosed animals accumulated bound MC-LR in liver tissue, averaging 26.4 μg, approximately 1.1{\%} of the dose administered. These results point to the potential uptake and accumulation of MC-LR in human liver tissue exposed chronically to sub-acute doses.",
author = "Brett Greer and Meneely, {Julie P.} and Elliott, {Christopher T.}",
year = "2018",
month = "3",
day = "20",
doi = "10.1038/s41598-018-23312-7",
language = "English",
volume = "8",
journal = "Nature Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Uptake and accumulation of Microcystin-LR based on exposure through drinking water: An animal model assessing the human health risk

AU - Greer, Brett

AU - Meneely, Julie P.

AU - Elliott, Christopher T.

PY - 2018/3/20

Y1 - 2018/3/20

N2 - Harmful Algal Blooms (HABs) in freshwater systems and intensified aquaculture have increased the risk to human health through exposure to cyanotoxins such as microcystin-LR (MC-LR). To understand the uptake and processing of MC-LR in humans, the pig was chosen as an animal model. This was assessed by repeated exposure for 13 weeks of eight animals dosed daily with MC-LR at 0.04 μg/kg bw, repeated with six animals over five weeks at a dose 50 times higher at 2 μg/kg bw. An analytical method was developed for MC-LR in porcine serum and also to analyse levels of free MC-LR in harvested porcine tissues, with Lemieux Oxidation employed to determine bound MC-LR in these tissues. MC-LR was not detected in the serum of treated animals from either experiment but free MC-LR was observed in the large intestine and kidney from two animals from the higher dosed group at levels of 1.4 and 1.9 μg/kg dry weight (dw) respectively. The results indicated 50% of higher dosed animals accumulated bound MC-LR in liver tissue, averaging 26.4 μg, approximately 1.1% of the dose administered. These results point to the potential uptake and accumulation of MC-LR in human liver tissue exposed chronically to sub-acute doses.

AB - Harmful Algal Blooms (HABs) in freshwater systems and intensified aquaculture have increased the risk to human health through exposure to cyanotoxins such as microcystin-LR (MC-LR). To understand the uptake and processing of MC-LR in humans, the pig was chosen as an animal model. This was assessed by repeated exposure for 13 weeks of eight animals dosed daily with MC-LR at 0.04 μg/kg bw, repeated with six animals over five weeks at a dose 50 times higher at 2 μg/kg bw. An analytical method was developed for MC-LR in porcine serum and also to analyse levels of free MC-LR in harvested porcine tissues, with Lemieux Oxidation employed to determine bound MC-LR in these tissues. MC-LR was not detected in the serum of treated animals from either experiment but free MC-LR was observed in the large intestine and kidney from two animals from the higher dosed group at levels of 1.4 and 1.9 μg/kg dry weight (dw) respectively. The results indicated 50% of higher dosed animals accumulated bound MC-LR in liver tissue, averaging 26.4 μg, approximately 1.1% of the dose administered. These results point to the potential uptake and accumulation of MC-LR in human liver tissue exposed chronically to sub-acute doses.

UR - http://www.scopus.com/inward/record.url?scp=85044299975&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-23312-7

DO - 10.1038/s41598-018-23312-7

M3 - Article

AN - SCOPUS:85044299975

VL - 8

JO - Nature Scientific Reports

JF - Nature Scientific Reports

SN - 2045-2322

IS - 1

M1 - 4913

ER -