Abstract
Harmful Algal Blooms (HABs) in freshwater systems and intensified aquaculture have increased the risk to human health through exposure to cyanotoxins such as microcystin-LR (MC-LR). To understand the uptake and processing of MC-LR in humans, the pig was chosen as an animal model. This was assessed by repeated exposure for 13 weeks of eight animals dosed daily with MC-LR at 0.04 μg/kg bw, repeated with six animals over five weeks at a dose 50 times higher at 2 μg/kg bw. An analytical method was developed for MC-LR in porcine serum and also to analyse levels of free MC-LR in harvested porcine tissues, with Lemieux Oxidation employed to determine bound MC-LR in these tissues. MC-LR was not detected in the serum of treated animals from either experiment but free MC-LR was observed in the large intestine and kidney from two animals from the higher dosed group at levels of 1.4 and 1.9 μg/kg dry weight (dw) respectively. The results indicated 50% of higher dosed animals accumulated bound MC-LR in liver tissue, averaging 26.4 μg, approximately 1.1% of the dose administered. These results point to the potential uptake and accumulation of MC-LR in human liver tissue exposed chronically to sub-acute doses.
| Original language | English |
|---|---|
| Article number | 4913 |
| Number of pages | 10 |
| Journal | Scientific Reports |
| Volume | 8 |
| Issue number | 1 |
| Early online date | 20 Mar 2018 |
| DOIs | |
| Publication status | Early online date - 20 Mar 2018 |
Fingerprint
Cite this
}
Uptake and accumulation of Microcystin-LR based on exposure through drinking water: An animal model assessing the human health risk. / Greer, Brett; Meneely, Julie P.; Elliott, Christopher T.
In: Scientific Reports, Vol. 8, No. 1, 4913, 20.03.2018.Research output: Contribution to journal › Article
TY - JOUR
T1 - Uptake and accumulation of Microcystin-LR based on exposure through drinking water: An animal model assessing the human health risk
AU - Greer, Brett
AU - Meneely, Julie P.
AU - Elliott, Christopher T.
PY - 2018/3/20
Y1 - 2018/3/20
N2 - Harmful Algal Blooms (HABs) in freshwater systems and intensified aquaculture have increased the risk to human health through exposure to cyanotoxins such as microcystin-LR (MC-LR). To understand the uptake and processing of MC-LR in humans, the pig was chosen as an animal model. This was assessed by repeated exposure for 13 weeks of eight animals dosed daily with MC-LR at 0.04 μg/kg bw, repeated with six animals over five weeks at a dose 50 times higher at 2 μg/kg bw. An analytical method was developed for MC-LR in porcine serum and also to analyse levels of free MC-LR in harvested porcine tissues, with Lemieux Oxidation employed to determine bound MC-LR in these tissues. MC-LR was not detected in the serum of treated animals from either experiment but free MC-LR was observed in the large intestine and kidney from two animals from the higher dosed group at levels of 1.4 and 1.9 μg/kg dry weight (dw) respectively. The results indicated 50% of higher dosed animals accumulated bound MC-LR in liver tissue, averaging 26.4 μg, approximately 1.1% of the dose administered. These results point to the potential uptake and accumulation of MC-LR in human liver tissue exposed chronically to sub-acute doses.
AB - Harmful Algal Blooms (HABs) in freshwater systems and intensified aquaculture have increased the risk to human health through exposure to cyanotoxins such as microcystin-LR (MC-LR). To understand the uptake and processing of MC-LR in humans, the pig was chosen as an animal model. This was assessed by repeated exposure for 13 weeks of eight animals dosed daily with MC-LR at 0.04 μg/kg bw, repeated with six animals over five weeks at a dose 50 times higher at 2 μg/kg bw. An analytical method was developed for MC-LR in porcine serum and also to analyse levels of free MC-LR in harvested porcine tissues, with Lemieux Oxidation employed to determine bound MC-LR in these tissues. MC-LR was not detected in the serum of treated animals from either experiment but free MC-LR was observed in the large intestine and kidney from two animals from the higher dosed group at levels of 1.4 and 1.9 μg/kg dry weight (dw) respectively. The results indicated 50% of higher dosed animals accumulated bound MC-LR in liver tissue, averaging 26.4 μg, approximately 1.1% of the dose administered. These results point to the potential uptake and accumulation of MC-LR in human liver tissue exposed chronically to sub-acute doses.
UR - http://www.scopus.com/inward/record.url?scp=85044299975&partnerID=8YFLogxK
U2 - 10.1038/s41598-018-23312-7
DO - 10.1038/s41598-018-23312-7
M3 - Article
AN - SCOPUS:85044299975
VL - 8
JO - Nature Scientific Reports
JF - Nature Scientific Reports
SN - 2045-2322
IS - 1
M1 - 4913
ER -