Use of antigen mimics to produce specific antibodies to anti-coccidial drugs

Terence Fodey, P. Delahaut, Christopher Elliott

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A range of polyclonal antibodies was successfully produced to the coccidiostatic drugs diclazuril and robenidine. Initial attempts to make immunogenic complexes of both drugs were ineffective due to difficulties encountered while trying to couple the compounds to large carrier proteins. Structural mimics, which could act as haptens for each drug, were sought and identified. The compounds identified were more open to chemical manipulation and were conjugated to carrier proteins to produce effective immunogens. The most sensitive antisera produced displayed IC(50)s of 1.5 ng/ml and 13 ng/ml for diclazuril and robenidine respectively. The antibody for diclazuril was shown to be specific, cross-reacting only with clazuril by 15%. The robenidine antibody displayed a low cross-reactivity of 1.2% to the compound used to produce the antibody. (C) 2007 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalJournal of Immunological Methods
Volume323
Issue number1
DOIs
Publication statusPublished - 31 May 2007

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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