Use of immunosorbent-purified antigens of Fasciola hepatica in enzyme immunoassays

A Trudgett, A Anderson, R E Hanna

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A monoclonal antibody specific for the T1 tegumental antigen of Fasciola hepatica was used as a solid-phase immunosorbent for the purification of T1 antigen from homogenised mature F hepatica. Material fractionated by this technique was successfully used in enzyme-linked immunoassays to detect antibodies to F hepatica in sera from sheep and cattle. Species differences in response to infection by F hepatica were demonstrated.
Original languageEnglish
Pages (from-to)262-3
Number of pages2
JournalResearch in Veterinary Science
Volume44
Issue number2
Publication statusPublished - Mar 1988

Keywords

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Helminth
  • Cattle
  • Cattle Diseases
  • Enzyme-Linked Immunosorbent Assay
  • Fasciola hepatica
  • Fascioliasis
  • Immunosorbent Techniques
  • Sheep
  • Sheep Diseases

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