Validation of the CDC Biofilm Reactor as a Dynamic Model for Assessment of Encrustation Formation on Urological Device Materials

Brendan Gilmore, Terence Hamill, David Jones, Sean Gorman

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Contemporary medical science is reliant upon the rational selection and utilization of devices, and therefore, an increasing need has developed for in vitro systems aimed at replicating the conditions to which urological devices will be subjected to during their use in vivo. We report the development and validation of a novel continuous flow encrustation model based on the commercially available CDC biofilm reactor. Proteus mirabilis-induced encrustation formation on test biomaterial sections under varying experimental parameters was analyzed by X-ray diffraction, infrared- and Raman spectroscopy and by scanning electron microscopy. The model system produced encrusted deposits similar to those observed in archived clinical samples. Results obtained for the system are highly reproducible with encrustation being rapidly deposited on test biomaterial sections. This model will have utility in the rapid screening of encrustation behavior of biomaterials for use in urological applications. (C) 2010 Wiley Periodicals. Inc. J Biomed Mater Res Part B: Appl Biomater 93B: 128-140, 2010
Original languageEnglish
Pages (from-to)128-140
Number of pages13
JournalJournal Of Biomedical Materials Research Part B-applied Biomaterials
Volume93B
Issue number1
DOIs
Publication statusPublished - Apr 2010

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

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