Vitamin D binding protein isoforms as candidate predictors of disease extension in childhood arthritis

David S. Gibson, Keri Newell, Alexandra N. Evans, Sorcha Finnegan, Gwen Manning, Caitriona Scaife, Catherine McAllister, Stephen R. Pennington, Mark W. Duncan, Terry L. Moore, Madeleine E. Rooney

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)
191 Downloads (Pure)

Abstract

Introduction: Juvenile idiopathic arthritis (JIA) comprises a poorly understood group of chronic autoimmune diseases with variable clinical outcomes. We investigated whether the synovial fluid (SF) proteome could distinguish a subset of patients in whom disease extends to affect a large number of joints.

Methods: SF samples from 57 patients were obtained around time of initial diagnosis of JIA, labeled with Cy dyes and separated by two-dimensional electrophoresis. Multivariate analyses were used to isolate a panel of proteins which distinguish patient subgroups. Proteins were identified using MALDI-TOF mass spectrometry with expression verified by immunochemical methods. Protein glycosylation status was confirmed by hydrophilic interaction liquid chromatography.

Results: A truncated isoform of vitamin D binding protein (VDBP) is present at significantly reduced levels in the SF of oligoarticular patients at risk of disease extension, relative to other subgroups (p < 0.05). Furthermore, sialylated forms of immunopurified synovial VDBP were significantly reduced in extended oligoarticular patients (p < 0.005).

Conclusion: Reduced conversion of VDBP to a macrophage activation factor may be used to stratify patients to determine risk of disease extension in JIA patients.
Original languageEnglish
Pages (from-to)5479-5492
Number of pages14
JournalJournal of proteomics
Volume75
Issue number17
Early online date05 Jul 2012
DOIs
Publication statusPublished - 18 Sep 2012

Keywords

  • Juvenile idiopathic arthritis
  • Proteomics
  • Synovial fluid
  • Vitamin D binding protein
  • Inflammation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

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