Water‐Soluble Triarylborane Chromophores for One‐and Two‐Photon Excited Fluorescence Imaging of Mitochondria in Cells

Stephanie Griesbeck, Zuolun Zhang, Marcus Gutmann, Tessa Luehmann, Robert M. Edkins, Guillaume Clermont, Adina N. Lazar, Martin Haehnel, Katharina Edkins, Antonius Eichhorn, Mireille Blanchard-Desce, Lorenz Meinel, Todd B. Marder

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47 Citations (Scopus)
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Abstract

Three water-soluble tetracationic quadrupolar chromophores comprising two three-coordinate boron π-acceptor groups bridged by thiophene-containing moieties were synthesised for biological imaging applications. Compound 3 containing the bulkier 5-(3,5-Me2C6H2)-2,2′-(C4H2S)2-5′-(3,5-Me2C6H2) bridge is stable over a long period of time, exhibits a high fluorescence quantum yield and strong one- and two-photon absorption (TPA), and has a TPA cross section of 268 GM at 800 nm in water. Confocal laser scanning fluorescence microscopy studies in live cells indicated localisation of the chromophore at the mitochondria; moreover, cytotoxicity measurements proved biocompatibility. Thus, chromophore 3 has excellent potential for one- and two-photon-excited fluorescence imaging of mitochondrial function in cells.
Original languageEnglish
Pages (from-to)14701-14706
Number of pages6
JournalChemistry - A European Journal
Volume22
Issue number41
Early online date15 Sep 2016
DOIs
Publication statusPublished - 04 Oct 2016

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