Whole-genome sequence of the bovine blood fluke Schistosoma bovis supports interspecific hybridization with S. haematobium

Harald Oey, Martha Zakrzewski, Kerstin Gravermann, Neil D Young, Pasi K Korhonen, Geoffrey N Gobert, Sujeevi Nawaratna, Shihab Hasan, David M Martínez, Hong You, Martin Lavin, Malcolm K Jones, Mark A Ragan, Jens Stoye, Ana Oleaga, Aidan M Emery, Bonnie Webster, David Rollinson, Robin B Gasser, Donald P McManus & 1 others Lutz Krause

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Intestinal infection by the parasitic blood fluke Schistosoma bovis is a common veterinary problem in Africa and the Middle East and occasionally in the Mediterranean Region. The species also has the ability to form interspecific hybrids with the human parasite S. haematobium with natural hybridisation observed in West Africa, presenting possible zoonotic transmission. Additionally, this exchange of alleles between species may dramatically influence disease dynamics and parasite evolution. We have generated a 374 Mb assembly of the S. bovis genome using Illumina and PacBio-based technologies. Despite infecting different hosts and organs, the genome sequences of S. bovis and S. haematobium appeared strikingly similar with 97% sequence identity. The two species share 98% of protein-coding genes, with an average sequence identity of 97.3% at the amino acid level. Genome comparison identified large continuous parts of the genome (up to several 100 kb) showing almost 100% sequence identity between S. bovis and S. haematobium. It is unlikely that this is a result of genome conservation and provides further evidence of natural interspecific hybridization between S. bovis and S. haematobium. Our results suggest that foreign DNA obtained by interspecific hybridization was maintained in the population through multiple meiosis cycles and that hybrids were sexually reproductive, producing viable offspring. The S. bovis genome assembly forms a highly valuable resource for studying schistosome evolution and exploring genetic regions that are associated with species-specific phenotypic traits.

LanguageEnglish
Article numbere1007513
JournalPLoS Pathogens
Volume15
Issue number1
Early online date23 Jan 2019
DOIs
Publication statusEarly online date - 23 Jan 2019
Externally publishedYes

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Schistosoma
Trematoda
Genome
Parasites
Mediterranean Region
Parasitic Diseases
Western Africa
Middle East
Molecular Evolution
Zoonoses
Meiosis
Alleles
Technology
Amino Acids
DNA
Population

Cite this

Oey, Harald ; Zakrzewski, Martha ; Gravermann, Kerstin ; Young, Neil D ; Korhonen, Pasi K ; Gobert, Geoffrey N ; Nawaratna, Sujeevi ; Hasan, Shihab ; Martínez, David M ; You, Hong ; Lavin, Martin ; Jones, Malcolm K ; Ragan, Mark A ; Stoye, Jens ; Oleaga, Ana ; Emery, Aidan M ; Webster, Bonnie ; Rollinson, David ; Gasser, Robin B ; McManus, Donald P ; Krause, Lutz. / Whole-genome sequence of the bovine blood fluke Schistosoma bovis supports interspecific hybridization with S. haematobium. In: PLoS Pathogens. 2019 ; Vol. 15, No. 1.
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abstract = "Intestinal infection by the parasitic blood fluke Schistosoma bovis is a common veterinary problem in Africa and the Middle East and occasionally in the Mediterranean Region. The species also has the ability to form interspecific hybrids with the human parasite S. haematobium with natural hybridisation observed in West Africa, presenting possible zoonotic transmission. Additionally, this exchange of alleles between species may dramatically influence disease dynamics and parasite evolution. We have generated a 374 Mb assembly of the S. bovis genome using Illumina and PacBio-based technologies. Despite infecting different hosts and organs, the genome sequences of S. bovis and S. haematobium appeared strikingly similar with 97{\%} sequence identity. The two species share 98{\%} of protein-coding genes, with an average sequence identity of 97.3{\%} at the amino acid level. Genome comparison identified large continuous parts of the genome (up to several 100 kb) showing almost 100{\%} sequence identity between S. bovis and S. haematobium. It is unlikely that this is a result of genome conservation and provides further evidence of natural interspecific hybridization between S. bovis and S. haematobium. Our results suggest that foreign DNA obtained by interspecific hybridization was maintained in the population through multiple meiosis cycles and that hybrids were sexually reproductive, producing viable offspring. The S. bovis genome assembly forms a highly valuable resource for studying schistosome evolution and exploring genetic regions that are associated with species-specific phenotypic traits.",
author = "Harald Oey and Martha Zakrzewski and Kerstin Gravermann and Young, {Neil D} and Korhonen, {Pasi K} and Gobert, {Geoffrey N} and Sujeevi Nawaratna and Shihab Hasan and Mart{\'i}nez, {David M} and Hong You and Martin Lavin and Jones, {Malcolm K} and Ragan, {Mark A} and Jens Stoye and Ana Oleaga and Emery, {Aidan M} and Bonnie Webster and David Rollinson and Gasser, {Robin B} and McManus, {Donald P} and Lutz Krause",
year = "2019",
month = "1",
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Oey, H, Zakrzewski, M, Gravermann, K, Young, ND, Korhonen, PK, Gobert, GN, Nawaratna, S, Hasan, S, Martínez, DM, You, H, Lavin, M, Jones, MK, Ragan, MA, Stoye, J, Oleaga, A, Emery, AM, Webster, B, Rollinson, D, Gasser, RB, McManus, DP & Krause, L 2019, 'Whole-genome sequence of the bovine blood fluke Schistosoma bovis supports interspecific hybridization with S. haematobium', PLoS Pathogens, vol. 15, no. 1, e1007513. https://doi.org/10.1371/journal.ppat.1007513

Whole-genome sequence of the bovine blood fluke Schistosoma bovis supports interspecific hybridization with S. haematobium. / Oey, Harald; Zakrzewski, Martha; Gravermann, Kerstin; Young, Neil D; Korhonen, Pasi K; Gobert, Geoffrey N; Nawaratna, Sujeevi; Hasan, Shihab; Martínez, David M; You, Hong; Lavin, Martin; Jones, Malcolm K; Ragan, Mark A; Stoye, Jens; Oleaga, Ana; Emery, Aidan M; Webster, Bonnie; Rollinson, David; Gasser, Robin B; McManus, Donald P; Krause, Lutz.

In: PLoS Pathogens, Vol. 15, No. 1, e1007513, 23.01.2019.

Research output: Contribution to journalArticle

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T1 - Whole-genome sequence of the bovine blood fluke Schistosoma bovis supports interspecific hybridization with S. haematobium

AU - Oey, Harald

AU - Zakrzewski, Martha

AU - Gravermann, Kerstin

AU - Young, Neil D

AU - Korhonen, Pasi K

AU - Gobert, Geoffrey N

AU - Nawaratna, Sujeevi

AU - Hasan, Shihab

AU - Martínez, David M

AU - You, Hong

AU - Lavin, Martin

AU - Jones, Malcolm K

AU - Ragan, Mark A

AU - Stoye, Jens

AU - Oleaga, Ana

AU - Emery, Aidan M

AU - Webster, Bonnie

AU - Rollinson, David

AU - Gasser, Robin B

AU - McManus, Donald P

AU - Krause, Lutz

PY - 2019/1/23

Y1 - 2019/1/23

N2 - Intestinal infection by the parasitic blood fluke Schistosoma bovis is a common veterinary problem in Africa and the Middle East and occasionally in the Mediterranean Region. The species also has the ability to form interspecific hybrids with the human parasite S. haematobium with natural hybridisation observed in West Africa, presenting possible zoonotic transmission. Additionally, this exchange of alleles between species may dramatically influence disease dynamics and parasite evolution. We have generated a 374 Mb assembly of the S. bovis genome using Illumina and PacBio-based technologies. Despite infecting different hosts and organs, the genome sequences of S. bovis and S. haematobium appeared strikingly similar with 97% sequence identity. The two species share 98% of protein-coding genes, with an average sequence identity of 97.3% at the amino acid level. Genome comparison identified large continuous parts of the genome (up to several 100 kb) showing almost 100% sequence identity between S. bovis and S. haematobium. It is unlikely that this is a result of genome conservation and provides further evidence of natural interspecific hybridization between S. bovis and S. haematobium. Our results suggest that foreign DNA obtained by interspecific hybridization was maintained in the population through multiple meiosis cycles and that hybrids were sexually reproductive, producing viable offspring. The S. bovis genome assembly forms a highly valuable resource for studying schistosome evolution and exploring genetic regions that are associated with species-specific phenotypic traits.

AB - Intestinal infection by the parasitic blood fluke Schistosoma bovis is a common veterinary problem in Africa and the Middle East and occasionally in the Mediterranean Region. The species also has the ability to form interspecific hybrids with the human parasite S. haematobium with natural hybridisation observed in West Africa, presenting possible zoonotic transmission. Additionally, this exchange of alleles between species may dramatically influence disease dynamics and parasite evolution. We have generated a 374 Mb assembly of the S. bovis genome using Illumina and PacBio-based technologies. Despite infecting different hosts and organs, the genome sequences of S. bovis and S. haematobium appeared strikingly similar with 97% sequence identity. The two species share 98% of protein-coding genes, with an average sequence identity of 97.3% at the amino acid level. Genome comparison identified large continuous parts of the genome (up to several 100 kb) showing almost 100% sequence identity between S. bovis and S. haematobium. It is unlikely that this is a result of genome conservation and provides further evidence of natural interspecific hybridization between S. bovis and S. haematobium. Our results suggest that foreign DNA obtained by interspecific hybridization was maintained in the population through multiple meiosis cycles and that hybrids were sexually reproductive, producing viable offspring. The S. bovis genome assembly forms a highly valuable resource for studying schistosome evolution and exploring genetic regions that are associated with species-specific phenotypic traits.

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DO - 10.1371/journal.ppat.1007513

M3 - Article

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JO - PLoS Pathogens

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JF - PLoS Pathogens

SN - 1553-7366

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