Methods: Sputum samples (n=95) were collected from PWCF (n=19) enrolled to the CFMATTERS study (Cystic Fibrosis Microbiome-determined Antibiotic Therapy Trial in Exacerbations: Results Stratified) at baseline (day 0) and subsequently at three monthly intervals for up-to 12 months (month 3, month 6, month 9 and month 12) in absence of PEx. Genomic DNA was extracted and microbial community profiles determined by sequencing the 16S rRNA marker gene using the Illumina MiSeq platform. Microbial community composition was determined as relative abundance (RA) of the corresponding taxa and alpha-diversity (taxonomic richness, Shannon-Wiener diversity, evenness and dominance) was calculated for each sample.
Results: Over a period of one year, there was no significant differences in lung function (FEV1 % of predicted [p=0.997] and FVC % of predicted [p=0.977], respectively) during clinical stability. Some intra-patient variability was observed between visits; however, overall community composition remained stable. No difference was observed in RA for the main aerobic taxa (Pseudomonas spp. [p=0.857]; Streptococcus spp. [p=0.888], Staphylococcus spp. [p=0.918]) and obligate anaerobic taxa (Prevotella spp. [p=0.977]; Veillonella spp. [p=0.314]; Porphyromonas spp. [p=0.513]). Similarly, there was no difference observed for the main ecological measurements of community richness (p=0.835), Shannon-Wiener diversity (p=0.974), evenness (p=0.722) and dominance (p=0.987).
Conclusion: Despite some intra-patient variability, during periods of clinical stability, microbial community composition remains stable. Similarly, alpha-diversity measurements show little change over time.
Funding: CFMATTERS (603038.)
|Title of host publication||Journal of Cysric Fibrosis|
|Publisher||European Cystic Fibrosis Society|
|Number of pages||1|
|Publication status||Published - 06 Jun 2019|
|Event|| 42nd CF Conference. 05-08 June 2019, Liverpool, United Kingdom - Liverpool, United Kingdom|
Duration: 05 Jun 2019 → 08 Jun 2019
Conference number: 42nd
|Conference||42nd CF Conference. 05-08 June 2019, Liverpool, United Kingdom|
|Period||05/06/2019 → 08/06/2019|