Analysis of a Novel Antimicrobial Peptide QUB-1746 from the Skin Secretion of the Frog, Kassina senegalensis

  • Hui Wu

Student thesis: Masters ThesisMaster of Philosophy

Abstract

It is well-known that the skin secretions of amphibians contain abundant bioactive compounds, such as biogenic amines, steroids, alkaloids, peptides and proteins. In this thesis, the full-length cDNA sequence of a novel antimicrobial peptide, named QUB-1746, was cloned from a Kassina senegalensis skin secretion derived cDNA library through ‘shotgun’ cloning. The mature peptide was synthesized by solid phase peptide synthesis (SPPS). RP-HPLC and MALDI-TOF MS were used to purify and analyze the mature peptide QUB-1746. Its minimal inhibitory concentration (MIC) was detected by using different micro-organisms and its haemolytic activity was tested by using horse red blood cells. MTT assay tested two human lung cancer cell lines, H23 and H157, for effects. The results of antimicrobial experiments showed that QUB-1746 was active only against the Gram-positive bacterium, S.aureus with an MIC value of 32 μM. The results of MTT assays showed that QUB-1746 had inhibitory activity against human lung cancer cells, H23 with an IC50 of 40.3 μM. Also, QUB-1746 possessed little haemolytic activity at less than 20% at 512 μM.
Date of AwardDec 2019
Original languageEnglish
Awarding Institution
  • Queen's University Belfast
SupervisorTianbao Chen (Supervisor), Mei Zhou (Supervisor) & Lei Wang (Supervisor)

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