AbstractBioactive peptide isolated from the granular glands of frog skin have been widely discovered to exhibit antimicrobial and anticancer activities. Since the antibiotic resistance has become a severe difficulty in modern therapeutic treatment, many of them have been developed into clinical application for the treatment of microbial infection and cancer.
In this thesis, a potent natural antimicrobial peptide QUB-1433 was identified and cloned from the skin secretion of Pelophylax nigromaculatus via “shotgun” cloning. According to the amino acid residues of QUB-1433, the pure peptide was synthesised and purified via solid-phase peptide synthesis and RP-HPLC. Subsequently, antimicrobial, anticancer and haemolysis assays were performed on QUB-1433 for its functional evaluation. From the results of a series of bioactive assays, QUB-1433 displayed activities of against Gram-positive bacteria and fungi (MIC values are 16 and 32 µM), but is lack in broad-spectrum of anticancer ability (MIC value is 128 µM). Moreover, QUB-1433 possesses relative low cytotoxicity towards mammalian cell. Hence the bioactivities of QUB-1433 provided new insight in the discovery of new drugs. However, the mechanisms of action and optimization of QUB-1433 still required the further exploration.
|Date of Award||Dec 2019|
|Supervisor||Tianbao Chen (Supervisor), Lei Wang (Supervisor), Mei Zhou (Supervisor) & Chengbang Ma (Supervisor)|