Abstract
Problems with steroid analysis in a clinical lab have been well characterized over the years, and organizations have issued position statements and recommendations on androgen-disorder diagnostics. This research aims to discover and develop an antibody highly specific for testosterone with minimal cross-reactivity to DHEA and other steroids using a combination of classical hybridoma technologies and in silico engineering techniques. By assessing the docking energies of testosterone and other steroids to the selected antibody molecular dynamics was employed to observe potential conformational changes in the binding process. This information will aid in further developing the antibody for affinity maturation and specificity optimization.Thesis is embargoed until 31 December 2028.
| Date of Award | Dec 2023 |
|---|---|
| Original language | English |
| Awarding Institution |
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| Sponsors | Fusion Antibodies plc |
| Supervisor | Roberta Burden (Supervisor) & Christopher Scott (Supervisor) |
Keywords
- Antibodies
- diagnostics
- steroids
- antibody engineering
- computational biology
- computational methods