Identification, isolation and bioactivity assessment of a bioactive peptide QUB-1465 from the skin secretion of Hylarana guentheri

Abstract

Amphibian skin secretions are a rich source of bioactive peptides, some of which can be developed as drug candidates. Among these natural peptides, AMPs have attracted much attention because of its potential to replace traditional antibiotics and solve the problem of microbial resistance as AMPs kill microorganisms through a membrane degradation mechanism, as described in the “barrel-stave-pore model”, the “toroidal-pore model” and the “carpet-like model”. In this research, a single cDNA sequence encoding a bioactive peptide QUB-1465 was cloned from a cDNA library constructed from the lyophilised secretion of Hylarana guentheri. A replijavascript:void(0);cate of peptide was produced through solid phase peptide synthesis. By use of RP- HPLC and MALDI-TOF MS, the synthesised peptide was purified and its mass charge ratio was further confirmed. QUB-1465 was found to inhibit the proliferation of microorganisms and cancer cell lines. QUB-1465 displayed antimicrobial activities against S. aureus（ATCC 10788）with MIC of 8 µM and MBC of 16 µM, and C.albicans (ATCC 10231) with MIC of 32 µM and MBC of 64 µM respectively. Also, its haemolytic activity was low with HC50 of 1.888mM. So QUB-1465 is promising antibacterial drug candidate.

Date of Award	Dec 2020
Original language	English
Awarding Institution	Queen's University Belfast
Supervisor	Mei Zhou (Supervisor), Lei Wang (Supervisor) & Chengbang Ma (Supervisor)