Microorganisms contribute to human welfare in a variety of ways. The microbial involvement in agriculture is crucial since it involves biocontrol, nutrient recycling to improve plant stress tolerance, and amelioration of soil faults. In agricultural microbiology, microbes that increase phosphorus abundance remain a major subject of study. The various microbial solubilisation mechanisms found in microorganisms play a significant part in the worldwide cycling of insoluble organic and inorganic phosphate. The goal of this study is to identify and isolate the bacteria that are found in alpine soils that can solubilise cerium phosphate. On NBRIP, a phosphate-free medium, bacteria were isolated, and over the course of 34 days, the growth curves were ascertained. The cultures were continuously cultivated using methanol as a carbon source. Two isolates (SB1 and SB8) from the alpine paleosols were found to solubilise phosphate from CePO
4. The isolates were identified as
Stenotrophomonas maltophilia (SB1) and
Bradyrhizobium sp (SB8). These bacteria's genomes underwent additional functional annotation, which made it possible to use state-of-the-art instruments like AlphaFold for in-depth structural analysis. Consequently, the XoxF and MxaF genes were modelled in three dimensions, providing insightful visualisations of their active regions. PyMOL played a key role in this approach by supporting the in-depth analysis of these gene areas.
Date of Award | Jul 2024 |
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Original language | English |
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Awarding Institution | - Queen's University Belfast
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Sponsors | Petroleum Technology Development Fund |
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Supervisor | Christopher C R Allen (Supervisor) & Deepak Kumaresan (Supervisor) |
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- Phosphate-solubilisation
- NBRIP
- cerium phosphate
- AlphaFold
- PyMOL
- XoxF
- MxaF
Isolation and characterisation of cerium phosphate solubilising bacteria from Alpine paleosols
Bashir Usman, S. (Author). Jul 2024
Student thesis: Doctoral Thesis › Doctor of Philosophy