AbstractAs antimicrobial resistance has become a threat towards human health for decades, the demand for the development of an alternative, broad-spectrum antimicrobial agent has turned into increasingly essential. As a consequence, the emergence of the antimicrobial peptide (AMP) derived from the skin secretion of amphibians has promoted the development of novel potential clinical therapeutics. Therefore, a novel potential bioactive peptide QUB-1475 derived from the frog species Hylarana guentheri was chosen here to get thoroughly studied.
In this research, the novel temporin-like peptide, with the sequence: FLQHIIGALGFIF-NH2, was discovered in the skin secretion of Hylarana guentheri. This thesis aimed to isolate and characterise the structure of the novel peptide QUB-1475 through “shotgun” molecular cloning, reverse-phase High-Performance Liquid Chromatography (RP-HPLC) and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) analysis. Then the peptide was chemically-synthesised and evaluated through a series of bioactivity assays.
This peptide displayed no antimicrobial activity on S. aureus, E. coli and C. albicans conducted in this research. The IC50 values of the peptide towards NCI-H23 cell line, PC3 cell line and U-251 MG cell line are 26.51 μM, 10.03 μM, and 44.78 μM respectively. Moreover, it should be emphasised that the novel peptide presented haemolytic rate of 18.95% at the concentration of 512 μM.
|Date of Award||Dec 2019|
|Supervisor||Tianbao Chen (Supervisor), Mei Zhou (Supervisor), Lei Wang (Supervisor) & Chengbang Ma (Supervisor)|