QUB-1957

: A bioactive peptide from the frog skin secretion of Hylarana latouchii

Abstract

The skin secretions of amphibians are significant for amphibians’ daily protection, especially the antimicrobial peptides. In this study, a cDNA library from lyophilised skin secretion of Hylarana latouchii was constructed, and molecular cloning of biosynthetic precursor-encoding cDNAs of QUB-1957 was performed. Subsequently, QUB-1957 was synthesised by Solid Phase Peptide Synthesizer. In the antimicrobial assays, the MIC of QUB-1957 against Gram-positive bacteria Staphylococcus aureus, Gram-negative bacteria Escherichia coli and yeast Candida albicans are 8 μM, 16 μM and 16 μM, respectively. Unfortunately, QUB-1957 also exhibits a certain degree of haemolytic rate, the HC50 is 47.82 μM. Besides, QUB-1957 exhibits no significant effect against PC3 (human prostate cancer cell line), NCI-H157 (human non-small cell lung cancer cell line), MCF-7 (human breast cancer cell line) and U251MG (human neuronal glioblastoma cell line). These characteristics of QUB-1957 indicate that QUB-1957 is a promising antimicrobial agent with a broad spectrum that worth further study. Hopefully, the structure QUB-1957 could be researched for novel antimicrobial agent development.

Date of Award	Jul 2018
Original language	English
Awarding Institution	Queen's University Belfast
Supervisor	Xinping Xi (Supervisor), Tianbao Chen (Supervisor), Mei Zhou (Supervisor), Lei Wang (Supervisor) & Christopher Shaw (Supervisor)