The discovery, identification, and bioactivity screening of peptide, QUB-2335, from skin secretion of the frog, Amolops hainanensis

  • Wenjia Zhao

Student thesis: Masters ThesisMaster of Philosophy

Abstract

Due to the resistance of antibiotics, the research of antimicrobial peptides has become a hot topic. In this study, a novel peptide named, QUB-2335, was identified and characterised from the skin secretion of the frog (Amolops hainanensis) by the strategy of ‘shotgun’ cloning. The deduced mature peptide sequence was confirmed as: FALGAVTKRLPSLFCLITRKC. QUB-2335 showed a broad-spectrum antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria, and yeast. The result of QUB-2335 towards E. coli and S. aureus in MIC assays, were 4 μM and 4 μM, and in MBC assays, were 16 μM and 8 μM. Also, the MIC/MBC values of QUB-2335 against C. albicans, were is 64 μM and 128 μM. The haemolysis assay results showed that the QUB-2335 had only 27.1% haemolysis at the concentration of 256μM. The anticancer cell assay revealed a potent inhibition of cancer cell proliferation. At a concentration of 10-4 M, a significant impact of QUB-2335 was observed. These experiments on QUB-2335 revealed antibacterial and anticancer cell activities with potential of low side effects. Thus, this peptide has some value and potential to become a clinical drug after further research and modification.

Thesis embargoed until 31st December 2027
Date of AwardDec 2022
Original languageEnglish
Awarding Institution
  • Queen's University Belfast
SupervisorMei Zhou (Supervisor), Tianbao Chen (Supervisor) & Yangyang Jiang (Supervisor)

Keywords

  • Antimicrobial peptides (AMPs)
  • antibiotic resistance
  • Hainanenin
  • amphibian skin secretion
  • QUB-2335

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