The use of reverse genetics to investigate plant-parasite interactions

Abstract

This thesis demonstrates the development and use of a novel Virus Induced Gene Silencing platform to investigate the interactions of plants and parasites. Host and parasite genes are targeted using this technology which when silenced indicate their involvement in establishing pathogenicity. The several root-expressed transporter proteins and Ethylene Response Factor genes in tomato were shown to be influential in the composition of Plant Root Exudate as well as the attraction and hatching of different plant nematode species. Silencing of the Phytoene Desaturase gene in the holoparasitic plant Cuscuta campestris was also shown to hinder growth, branching and a reduce the production of tissue carotenoid biosynthesis. Taken together the results of this thesis offer new avenues for targeted treatment of globally relevant crop pests and lay the groundwork for further study.

Thesis embargoed until 31 July 2023.

Date of Award	Jul 2022
Original language	English
Awarding Institution	Queen's University Belfast
Sponsors	Northern Ireland Department for the Economy
Supervisor	Aaron Maule (Supervisor) & Jonathan Dalzell (Supervisor)