Biological activities of cationicity-enhanced and hydrophobicity-optimized analogues of an antimicrobial peptide, dermaseptin-PS3, from the skin secretion of Phyllomedusa sauvagii

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    The skin secretions of the subfamily Phyllomedusinae have long been known to contain a number of compounds with antimicrobial potential. Herein, a biosynthetic dermaseptin-precursor cDNA was obtained from a Phyllomedusa sauvagii skin secretion-derived cDNA library, and thereafter, the presence of the mature peptide, namely dermaseptin-PS3 (DPS3), was confirmed by LC–MS/MS. Moreover, this naturally occurring peptide was utilized to design two analogues, K5, 17-DPS3 (introducing two lysine residues at positions 5 and 17 to replace acidic amino acids) and L10, 11-DPS3 (replacing two neutral amino acids with the hydrophobic amino acid, leucine), improving its cationicity on the polar/unipolar face and hydrophobicity in a highly conserved sequence motif, respectively. The results in regard to the two analogues show that either increasing cationicity, or hydrophobicity, enhance the antimicrobial activity. Also, the latter analogue had an enhanced anticancer activity, with pretreatment of H157 cells with 1 µM L10, 11-DPS3 decreasing viability by approximately 78%, even though this concentration of peptide exhibited no haemolytic effect. However, it must be noted that in comparison to the initial peptide, both analogues demonstrate higher membrane-rupturing capacity towards mammalian red blood cells

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    DOI

    Original languageEnglish
    JournalToxins
    Journal publication date07 Aug 2018
    Issue number8
    Volume10
    DOIs
    Publication statusPublished - 07 Aug 2018

      Research areas

    • antimicrobial peptide (AMP); dermaseptin; anuran skin secretion; drug design; antimicrobial activity; anticancer activity

    ID: 155829852