Catenins Steer Cell Migration via Stabilization of Front-Rear Polarity.

      Research output: Research - peer-reviewArticle

      Published
      • Vassil Vassilev
      • Anna Platek
      • Sylvain Hiver
      • Hideki Enomot
      • Masatoshi Takeich

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      Cell migration plays a pivotal role in morphogenetic and pathogenetic processes. To achieve directional migration, cells must establish a front-to-rear axis of polarity. Here we show that components of the cadherin-catenin complex function to stabilize this front-rear polarity. Neural crest and glioblastoma cells undergo directional migration in vivo or in vitro. During this process, αE-catenin accumulated at lamellipodial membranes and then moved toward the rear with the support of a tyrosine-phosphorylated β-catenin. This relocating αE-catenin bound to p115RhoGEF, leading to gathering of active RhoA in front of the nucleus where myosin-IIB arcs assemble. When catenins or p115RhoGEF were removed, cells lost the polarized myosin-IIB assembly, as well as the capability for directional movement. These results suggest that, apart from its well-known function in cell adhesion, the β-catenin/αE-catenin complex regulates directional cell migration by restricting active RhoA to perinuclear regions and controlling myosin-IIB dynamics at these sites.

      DOI

      Original languageEnglish
      Pages (from-to)463-479.e5
      JournalDevelopmental Cell
      Journal publication date20 Nov 2017
      Issue number4
      Volume43
      Early online date02 Nov 2017
      DOIs
      StatePublished - 20 Nov 2017

      ID: 137572210