Detection of tetrodotoxins in juvenile pufferfish Lagocephalus sceleratus (Gmelin, 1789) from the North Aegean Sea (Greece) by an electrochemical magnetic bead-based immunosensing tool

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    • Sandra Leonardo
    • Sotiris Kiparissis
    • Maria Rambla-Alegre
    • Sergi Almarza
    • Ana Roque
    • Karl B. Andree
    • Aris Christidis
    • Cintia Flores
    • Josep Caixach
    • Katrina Campbell
    • Christopher T. Elliott
    • Katerina Aligizaki
    • Jorge Diogène
    • Mònica Campàs

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    Two small Lagocephalus sceleratus juveniles were captured in picarel targeting catches from North Aegean Sea (Greece) in the autumn of 2017. An electrochemical immunosensing tool using magnetic beads as immobilisation support was developed and applied to the rapid screening of tetrodotoxins (TTXs), potent neurotoxins that constitute a food safety hazard when present in seafood. This tool revealed the presence of TTXs in both individuals. Results were compared with those provided by mELISA and LC-HRMS, the latter confirming the presence of TTX. Some of the tissues contained TTX contents close to or above 2 mg/kg. L. sceleratus juveniles had been considered as non-toxic and, to our knowledge, this is the first report of high TTX levels in small L. sceleratus individuals. Such specimens can be mistaken with other edible species, posing a threat to consumers. The availability of low-cost and user-friendly tools for TTXs detection will contribute to guarantee seafood safety.

    Documents

    • Detection of tetrodotoxins in juvenile pufferfish Lagocephalus sceleratus (Gmelin, 1789) from the North Aegean Sea (Greece) by an electrochemical magnetic bead-based immunosensing tool

      Rights statement: Copyright 2019 Elsevier. This manuscript is distributed under a Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits distribution and reproduction for non-commercial purposes, provided the author and source are cited.

      Accepted author manuscript, 877 KB, PDF-document

      Embargo ends: 29/03/2020

    DOI

    Original languageEnglish
    Pages (from-to)255 - 262
    JournalFood Chemistry
    Journal publication date30 Aug 2019
    Volume290
    Early online date29 Mar 2019
    DOIs
    Publication statusPublished - 30 Aug 2019

      Research areas

    • Tetrodotoxin, Electrochemical, Immunoassay, Magnetic bead, Liquid chromatography-high resolution mass spectrometry (LC-HRMS), Pufferfish, Juvenile,

    ID: 167126294