Helokinestatin-7 peptides from the venoms of Heloderma lizards

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    Helokinestatin-7 peptides from the venoms of Heloderma lizards. / Ma, Chengbang; Wang, Hui; Wu, Yuxin; Zhou, Mei; Lowe, Gemma; Wang, Lei; Zhang, Yingqi; Chen, Tianbao; Shaw, Christopher.

    In: Peptides, Vol. 35, No. 2, 06.2012, p. 300-305.

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    Ma, Chengbang ; Wang, Hui ; Wu, Yuxin ; Zhou, Mei ; Lowe, Gemma ; Wang, Lei ; Zhang, Yingqi ; Chen, Tianbao ; Shaw, Christopher. / Helokinestatin-7 peptides from the venoms of Heloderma lizards. In: Peptides. 2012 ; Vol. 35, No. 2. pp. 300-305.

    Bibtex

    @article{a7811108587b49a49f77669697b49e7f,
    title = "Helokinestatin-7 peptides from the venoms of Heloderma lizards",
    abstract = "Helokinestatins 1–6 constitute a family of bradykinin antagonist peptides originally isolated from the venoms of the Gila Monster, Heloderma suspectum and the Mexican beaded lizard, Heloderma horridum. Here we report the identification, isolation and preliminary pharmacological characterization of two novel tridecapeptides, named helokinestatin-7S (FDDDSTELILEPR – 1550 Da) and helokinestatin-7H (FDDDSRKLILEPR – 1604 Da), whose primary structures were predicted from cDNAs cloned from venom libraries of respective Heloderma lizards. Computed molecular masses of putative helokinestatin-7 peptides were used as tools to locate these peptides in archived LC/MS fractions from respective venoms and sequences were confirmed by MS/MS fragmentation. A synthetic replicate of helokinestatin-7H was found to antagonize the relaxation effect of bradykinin on rat arterial smooth muscle but to have no measurable effects alone. In contrast, synthetic helokinestatin-7S was found to directly contract this preparation. Studies on related natural peptides with subtle differences in primary structure can provide the tools for structure/activity studies in pharmacological investigations directed toward unraveling the molecular basis of venom toxicity and for the evaluation of potential therapeutic leads.Highlights► The paper describes two novel peptides, helokinestatin-7S and helokinestatin-7H. ► Helokinestatin-7S is from the venom of the Gila Monster (Heloderma suspectum). ► Helokinestatin-7H is from the venom of the Mexican beaded lizard (Heloderma horridum). ► Helokinestatin-7 peptides had different effects on rat tail artery smooth muscle.",
    author = "Chengbang Ma and Hui Wang and Yuxin Wu and Mei Zhou and Gemma Lowe and Lei Wang and Yingqi Zhang and Tianbao Chen and Christopher Shaw",
    year = "2012",
    month = "6",
    doi = "10.1016/j.peptides.2012.03.010",
    language = "English",
    volume = "35",
    pages = "300--305",
    journal = "Peptides",
    issn = "0196-9781",
    publisher = "Elsevier",
    number = "2",

    }

    RIS

    TY - JOUR

    T1 - Helokinestatin-7 peptides from the venoms of Heloderma lizards

    AU - Ma, Chengbang

    AU - Wang, Hui

    AU - Wu, Yuxin

    AU - Zhou, Mei

    AU - Lowe, Gemma

    AU - Wang, Lei

    AU - Zhang, Yingqi

    AU - Chen, Tianbao

    AU - Shaw, Christopher

    PY - 2012/6

    Y1 - 2012/6

    N2 - Helokinestatins 1–6 constitute a family of bradykinin antagonist peptides originally isolated from the venoms of the Gila Monster, Heloderma suspectum and the Mexican beaded lizard, Heloderma horridum. Here we report the identification, isolation and preliminary pharmacological characterization of two novel tridecapeptides, named helokinestatin-7S (FDDDSTELILEPR – 1550 Da) and helokinestatin-7H (FDDDSRKLILEPR – 1604 Da), whose primary structures were predicted from cDNAs cloned from venom libraries of respective Heloderma lizards. Computed molecular masses of putative helokinestatin-7 peptides were used as tools to locate these peptides in archived LC/MS fractions from respective venoms and sequences were confirmed by MS/MS fragmentation. A synthetic replicate of helokinestatin-7H was found to antagonize the relaxation effect of bradykinin on rat arterial smooth muscle but to have no measurable effects alone. In contrast, synthetic helokinestatin-7S was found to directly contract this preparation. Studies on related natural peptides with subtle differences in primary structure can provide the tools for structure/activity studies in pharmacological investigations directed toward unraveling the molecular basis of venom toxicity and for the evaluation of potential therapeutic leads.Highlights► The paper describes two novel peptides, helokinestatin-7S and helokinestatin-7H. ► Helokinestatin-7S is from the venom of the Gila Monster (Heloderma suspectum). ► Helokinestatin-7H is from the venom of the Mexican beaded lizard (Heloderma horridum). ► Helokinestatin-7 peptides had different effects on rat tail artery smooth muscle.

    AB - Helokinestatins 1–6 constitute a family of bradykinin antagonist peptides originally isolated from the venoms of the Gila Monster, Heloderma suspectum and the Mexican beaded lizard, Heloderma horridum. Here we report the identification, isolation and preliminary pharmacological characterization of two novel tridecapeptides, named helokinestatin-7S (FDDDSTELILEPR – 1550 Da) and helokinestatin-7H (FDDDSRKLILEPR – 1604 Da), whose primary structures were predicted from cDNAs cloned from venom libraries of respective Heloderma lizards. Computed molecular masses of putative helokinestatin-7 peptides were used as tools to locate these peptides in archived LC/MS fractions from respective venoms and sequences were confirmed by MS/MS fragmentation. A synthetic replicate of helokinestatin-7H was found to antagonize the relaxation effect of bradykinin on rat arterial smooth muscle but to have no measurable effects alone. In contrast, synthetic helokinestatin-7S was found to directly contract this preparation. Studies on related natural peptides with subtle differences in primary structure can provide the tools for structure/activity studies in pharmacological investigations directed toward unraveling the molecular basis of venom toxicity and for the evaluation of potential therapeutic leads.Highlights► The paper describes two novel peptides, helokinestatin-7S and helokinestatin-7H. ► Helokinestatin-7S is from the venom of the Gila Monster (Heloderma suspectum). ► Helokinestatin-7H is from the venom of the Mexican beaded lizard (Heloderma horridum). ► Helokinestatin-7 peptides had different effects on rat tail artery smooth muscle.

    U2 - 10.1016/j.peptides.2012.03.010

    DO - 10.1016/j.peptides.2012.03.010

    M3 - Article

    VL - 35

    SP - 300

    EP - 305

    JO - Peptides

    T2 - Peptides

    JF - Peptides

    SN - 0196-9781

    IS - 2

    ER -

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