Novel freeze-dried DDA and TPGS liposomes are suitable for nasal delivery of vaccine

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    There is a pressing need for effective needle-free vaccines that are stable enough for use in the developing world and stockpiling. The inclusion of the cationic lipid DDA and the PEG-containing moiety TPGS into liposomes has the potential to improve mucosal delivery. The aim of this study was to develop stable lyophilized cationic liposomes based on these materials suitable for nasal antigen delivery.

    Liposomes containing DDA and TPGS were developed. Size and zeta potential measurements, ex vivo, CLSM cell penetration study and cell viability investigations were made. Preliminary immunisation and stability studies using ovalbumin were performed.

    The liposomes exhibited suitable size and charge for permeation across nasal mucosa. DDA and TPGS increased tissue permeation in ex vivo studies and cell uptake with good cell viability. The liposomes improved immune response both locally and vaginally when compared to i.m administration or control liposomes delivered nasally. Additionally, the lyophilized products demonstrated good stability in terms of Tg, size and antigen retention.

    This study has shown that the novel liposomes have potential for development as a mucosal vaccine delivery system. Furthermore, the stability of the lyophilized liposomes offers potential additional benefits in terms of thermal stability over liquid formats.

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    • Novel freeze-dried DDA and TPGS liposomes are suitable for nasal delivery of vaccine

      Rights statement: © 2017 Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/,which permits distribution and reproduction for noncommercial purposes, provided the author and source are cited.

      Accepted author manuscript, 556 KB, PDF-document

    DOI

    Original languageEnglish
    JournalInternational Journal of Pharmaceutics
    Journal publication date06 Sep 2017
    Early online date06 Sep 2017
    DOIs
    Publication statusEarly online date - 06 Sep 2017

      Research areas

    • liposomes, nasal, TPGS, DDA, mucosal vaccine, temperature stability

    ID: 134580813