Quantitation of saxitoxin in human urine using immunocapture extraction and LC-MS

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    Aim: An immunomagnetic capture protocol for use with LC-MS was developed for the quantitation of saxitoxin (STX) in human urine. Materials & methods: This method uses monoclonal antibodies coupled to magnetic beads. STX was certified reference material grade from National Research Council, Canada. Analysis was carried out using LC-MS. Results: With an extraction efficiency of 80%, accuracy and precision of 93.0-100.2% and 5.3-12.6%, respectively, and a dynamic range of 1.00-100 ng/ml, the method is well suited to quantify STX exposures based on previously reported cases. Conclusion: Compared with our previously published protocols, this method has improved selectivity, a fivefold increase in sensitivity and uses only a third of the sample volume. This method can diagnose future toxin exposures and may complement the shellfish monitoring programs worldwide.

    DOI

    Original languageEnglish
    Number of pages11
    Pages (from-to)229-239
    JournalBioanalysis
    Journal publication dateFeb 2018
    Issue number4
    Volume10
    Early online date15 Jan 2018
    DOIs
    StatePublished - Feb 2018

      Research areas

    • antibody, immunocapture, liquid chromatography, magnetic beads, marine toxins, MS, saxitoxin

    ID: 140433207