Quantitation of saxitoxin in human urine using immunocapture extraction and LC-MS

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      AIM: An immunomagnetic capture protocol for use with LC-MS was developed for the quantitation of saxitoxin (STX) in human urine.

      MATERIALS & METHODS: This method uses monoclonal antibodies coupled to magnetic beads. STX was certified reference material grade from National Research Council, Canada. Analysis was carried out using LC-MS.

      RESULTS: With an extraction efficiency of 80%, accuracy and precision of 93.0-100.2% and 5.3-12.6%, respectively, and a dynamic range of 1.00-100 ng/ml, the method is well suited to quantify STX exposures based on previously reported cases.

      CONCLUSION: Compared with our previously published protocols, this method has improved selectivity, a fivefold increase in sensitivity and uses only a third of the sample volume. This method can diagnose future toxin exposures and may complement the shellfish monitoring programs worldwide.

      DOI

      Original languageEnglish
      JournalBioanalysis
      Journal publication date15 Jan 2018
      Early online date15 Jan 2018
      DOIs
      StateEarly online date - 15 Jan 2018

        Research areas

      • Journal Article

      ID: 140433207