Streptomyces albulus yields ε-poly-l-lysine and other products from salt-contaminated glycerol waste

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    Actinomycetes are the most important microorganisms for the industrial production of secondary metabolites with antimicrobial and anticancer properties. However, they have not been implicated in biorefineries. Here, we study the ability of the ε-poly-l-lysine producing Streptomyces albulus BCRC 11814 to utilize biodiesel-derived crude glycerol. S. albulus was cultured in a mineral medium supplemented with up to 10% w/v sodium chloride or potassium chloride, and with crude glycerol as the sole carbohydrate source. Under these conditions, the strain produced 0.1 g ε-poly-l-lysine per 1 g of biomass. RNA sequencing revealed upregulation of the ectoine biosynthetic pathway of S. albulus, which provides proof of halotolerance. S. albulus has several silent secondary metabolite biosynthetic clusters predicted within the genome. Based on the results, we conclude that S. albulus BCRC 11814 is a halotolerant microorganism capable of utilizing biodiesel-derived crude glycerol better than other actinomycetes included in the present study. S. albulus has the potential to be established as microbial platform production host for a range of high-value biological products.

    Original languageEnglish
    Pages (from-to)1-8
    JournalJournal of Industrial Microbiology and Biotechnology
    Journal publication date20 Sep 2018
    Early online date20 Sep 2018
    DOIs
    Publication statusEarly online date - 20 Sep 2018

      Research areas

    • Biodiesel, Biorefinery, Crude glycerol, Halotolerance, High-value products, Industrial biotechnology, RNA sequencing, Streptomyces albulus

    ID: 159764511