Uptake and accumulation of Microcystin-LR based on exposure through drinking water: An animal model assessing the human health risk

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    @article{40ab13fb035b4ece9ce91e6c8e172afb,
    title = "Uptake and accumulation of Microcystin-LR based on exposure through drinking water: An animal model assessing the human health risk",
    abstract = "Harmful Algal Blooms (HABs) in freshwater systems and intensified aquaculture have increased the risk to human health through exposure to cyanotoxins such as microcystin-LR (MC-LR). To understand the uptake and processing of MC-LR in humans, the pig was chosen as an animal model. This was assessed by repeated exposure for 13 weeks of eight animals dosed daily with MC-LR at 0.04 μg/kg bw, repeated with six animals over five weeks at a dose 50 times higher at 2 μg/kg bw. An analytical method was developed for MC-LR in porcine serum and also to analyse levels of free MC-LR in harvested porcine tissues, with Lemieux Oxidation employed to determine bound MC-LR in these tissues. MC-LR was not detected in the serum of treated animals from either experiment but free MC-LR was observed in the large intestine and kidney from two animals from the higher dosed group at levels of 1.4 and 1.9 μg/kg dry weight (dw) respectively. The results indicated 50{\%} of higher dosed animals accumulated bound MC-LR in liver tissue, averaging 26.4 μg, approximately 1.1{\%} of the dose administered. These results point to the potential uptake and accumulation of MC-LR in human liver tissue exposed chronically to sub-acute doses.",
    author = "Brett Greer and Meneely, {Julie P.} and Elliott, {Christopher T.}",
    year = "2018",
    month = "3",
    day = "20",
    doi = "10.1038/s41598-018-23312-7",
    language = "English",
    volume = "8",
    journal = "Nature Scientific Reports",
    issn = "2045-2322",
    publisher = "Nature Publishing Group",
    number = "1",

    }

    RIS

    TY - JOUR

    T1 - Uptake and accumulation of Microcystin-LR based on exposure through drinking water: An animal model assessing the human health risk

    AU - Greer, Brett

    AU - Meneely, Julie P.

    AU - Elliott, Christopher T.

    PY - 2018/3/20

    Y1 - 2018/3/20

    N2 - Harmful Algal Blooms (HABs) in freshwater systems and intensified aquaculture have increased the risk to human health through exposure to cyanotoxins such as microcystin-LR (MC-LR). To understand the uptake and processing of MC-LR in humans, the pig was chosen as an animal model. This was assessed by repeated exposure for 13 weeks of eight animals dosed daily with MC-LR at 0.04 μg/kg bw, repeated with six animals over five weeks at a dose 50 times higher at 2 μg/kg bw. An analytical method was developed for MC-LR in porcine serum and also to analyse levels of free MC-LR in harvested porcine tissues, with Lemieux Oxidation employed to determine bound MC-LR in these tissues. MC-LR was not detected in the serum of treated animals from either experiment but free MC-LR was observed in the large intestine and kidney from two animals from the higher dosed group at levels of 1.4 and 1.9 μg/kg dry weight (dw) respectively. The results indicated 50% of higher dosed animals accumulated bound MC-LR in liver tissue, averaging 26.4 μg, approximately 1.1% of the dose administered. These results point to the potential uptake and accumulation of MC-LR in human liver tissue exposed chronically to sub-acute doses.

    AB - Harmful Algal Blooms (HABs) in freshwater systems and intensified aquaculture have increased the risk to human health through exposure to cyanotoxins such as microcystin-LR (MC-LR). To understand the uptake and processing of MC-LR in humans, the pig was chosen as an animal model. This was assessed by repeated exposure for 13 weeks of eight animals dosed daily with MC-LR at 0.04 μg/kg bw, repeated with six animals over five weeks at a dose 50 times higher at 2 μg/kg bw. An analytical method was developed for MC-LR in porcine serum and also to analyse levels of free MC-LR in harvested porcine tissues, with Lemieux Oxidation employed to determine bound MC-LR in these tissues. MC-LR was not detected in the serum of treated animals from either experiment but free MC-LR was observed in the large intestine and kidney from two animals from the higher dosed group at levels of 1.4 and 1.9 μg/kg dry weight (dw) respectively. The results indicated 50% of higher dosed animals accumulated bound MC-LR in liver tissue, averaging 26.4 μg, approximately 1.1% of the dose administered. These results point to the potential uptake and accumulation of MC-LR in human liver tissue exposed chronically to sub-acute doses.

    UR - http://www.scopus.com/inward/record.url?scp=85044299975&partnerID=8YFLogxK

    U2 - 10.1038/s41598-018-23312-7

    DO - 10.1038/s41598-018-23312-7

    M3 - Article

    VL - 8

    JO - Nature Scientific Reports

    T2 - Nature Scientific Reports

    JF - Nature Scientific Reports

    SN - 2045-2322

    IS - 1

    M1 - 4913

    ER -

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